FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_242_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20939992
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG10600.016.27201531
CGACCGT13450.013.08052332
GTCCGAT27100.012.9085411
GTCCGTT23600.012.18123151
GTTCGAC14950.011.99861429
GTCCGCT26800.010.8559941
GTCGGAA33900.010.5235581
GTCGGTT16900.010.4522211
CCCGAAT23750.010.0626091
GTCGGAT26850.09.93279651
TCGACGC19300.09.83051843
TTCGACC19450.09.75479730
CGATCGG14550.09.72133941
GTCGTTT36900.09.57412051
CGCGTAA7759.276846E-119.34867
CGCGAGT9103.6379788E-129.09789443
AGTTCGA21400.09.02664928
GTCCGGA28600.08.9617081
TCGCGAG10050.08.92442842
GTCCGGT31200.08.8809711