FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_241_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_241_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18936238
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT21750.09.67548349
TCTCGAT23850.08.38969748
ATTCCGT26350.08.11715753
GATTCCG24400.08.0589452
ATCGACT29450.07.96362836
CGGTACA25750.07.90565161
TTCTCGA27050.07.524618647
GGCGGTA29150.07.338491459
TCGATTC29200.07.08861350
TAGATCG9402.5891313E-76.9713633
GTTTAAT88850.06.9277731
GCGTATA10503.441164E-86.90029539
TACATCG39400.06.7404123
GTAGTCG19500.06.7236357
GTTCGAT22150.06.7131421
GTTTTTT240750.06.63600641
GCGTAGT16251.8189894E-126.5802218
GTTTATT116050.06.49594641
TCGACTG26600.06.48296177
CGATTCC34650.06.47147151