FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_224_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_224_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16974946
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT19950.012.10463749
TCTCGAT24150.09.9996648
GATTCCG25200.09.719563552
TTCTCGA26550.09.22556547
TCGATTC25500.09.19936150
ATTCCGT26450.09.12980153
TCGCGAA6604.9582013E-88.88464720
CGGTACA26300.08.65892861
GGCGGTA25100.08.52272759
TCACCCG33450.08.4550933
ATCGCGA8555.7498255E-98.06875919
CGATTCC32450.07.76063251
CCGGACA34100.07.586242712
GCGGTAC29700.07.31914160
TTTCTCG34950.07.30397746
GTCTAAG31200.07.187021744
CCCGGAC37600.06.788082611
TAGCATA44900.06.75978959
GTTTAAT82450.06.66960051
ACCCGGC44850.06.5365635