FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_224_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16974946
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG11200.014.78406531
GTTCGAC12500.013.24636729
CGACCGT13200.013.06672632
GTCGGTA15150.012.57737351
TCGACGC15050.011.918797543
GTCCGTT21100.011.6577791
GTCGATT14450.011.26859951
GTCCGAT16850.011.1028321
TTCGACC15250.011.0840130
GTCCGCT23000.010.9960051
GTCCGGA24300.010.9780291
GTCGTTT28200.010.6883341
CCCGTAT16650.010.4038891
GTCGCTT25400.010.3662051
GTCGGTT17050.010.3630051
GTCGAAT13850.010.2559051
AGTTCGA17250.010.19838228
AAGTTCG15900.010.19649627
TCTCGAC17450.010.08280241
GTCGGAT18850.09.741021