FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_223_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_223_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15245663
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT18650.010.17401949
CGGTACA23400.08.69973661
GATTCCG21850.08.68377452
TCTCGAT20500.08.5828948
GGCGGTA22400.08.16376859
TCGATTC22350.07.872087550
GTCTAAG26600.07.522203444
ATTCCGT23850.07.52163453
TTCTCGA23000.07.499918547
TCACCCG29900.07.3821623
GCGGTAC25850.07.341284860
GTTTAAT68150.07.10403441
GTTTATT95500.06.8438641
TTCGCGG5050.00713390766.8320838
GGGAAAT93300.06.5604641
TAAATCG10552.7480746E-76.5381113
GTCGAAT8552.1684626E-56.47137831
CGTGGGT32600.06.45619357
TAGCATA40450.06.39470249
TTTCTCG29500.06.31481246