FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15245663
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT12400.018.7148081
TCGACCG8350.016.11198631
CGACCGT9900.013.58940132
GTTCGAC11350.012.4610129
GTCGCGT8600.012.0824511
GTCGGAA22200.012.0135121
GTCCGTT21300.011.5454531
GTCGCAT12600.011.5454521
CCCGAAT17400.011.3463941
CCCGTAT13150.011.3259571
GTCGTAT16300.010.8371431
TCGACGC13400.010.81209243
GCCGTAT13500.010.7757561
GTCCGCT18800.010.6856851
GTCCGCA21550.010.6078871
AAGTTCG14050.010.55699527
GTCGGAT17900.010.4489571
GGCGTTT24850.010.1748671
GTCCGGA20850.010.1334191
GGCGTAT15750.010.1160161