FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_222_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_222_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13760693
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT17250.09.99938649
GATTCCG18400.09.93674652
TCGATTC20250.09.19930250
ATTCCGT18650.08.87868253
GTTTAAT61300.08.1811481
TCTCGAT20550.08.05804648
TTCTCGA22750.08.0368747
TCGACTG18950.08.0082877
CGCTAGT5654.089837E-57.938027438
CGATTCC24300.07.80804951
CGGGAAT25850.06.95744041
TACATCG26150.06.8585043
GGAAAAT122600.06.65776161
CAACCGA16700.06.60885954
TCACCCG28900.06.563913
ACTAGGC14857.094059E-116.50349311
ATCGACT22350.06.481416
GTTGAAT54550.06.4037351
GTTTATT79750.06.37519361
GTAGTCG13653.075911E-96.31907457