FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13760693
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG7100.016.03381231
CGACCGT8200.014.72432532
GTCCGGT24400.012.2073031
GTCCGAA15050.011.2764061
GTCGAGA10050.011.0279561
GTTCGAC10100.010.92946129
GTCGTTT22350.010.8475491
GTCCGAT17300.010.6106241
GTCGTAT14200.010.4879651
GTCGGTT12350.010.376381
GTCGATT15750.010.1155251
TCGACGC11650.010.06738943
GTCCGTT18050.09.78597451
AAGTTCG12750.09.73958327
CGATCGG7555.4569682E-119.59588641
GTCCGCT17050.09.5473881
GTCGCTT19300.09.5110771
GTCGGTA19800.09.2708971
GTCGGCT20600.09.2471221
TTCGACC12750.09.19916330