FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_221_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_221_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4338214
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCCG6150.013.46277452
TCTCGAT6200.012.24163548
CTCGATT6500.012.20739349
TCGATTC7000.011.82800950
ATTCCGT6950.011.41672353
TTCTCGA8000.010.78098647
TATTCGC3351.2259651E-410.2955267
GCGTCGT5101.0184522E-710.14493819
CGATTCC8400.09.85667451
ATCGACT6302.3306711E-89.3068286
CCCGGAC9253.6379788E-128.94906811
GTTCGCT4007.0803676E-48.6447211
CGAAAAT6101.3144963E-68.5030051
TCACCCG9751.2732926E-118.4898483
TACATCG8951.7644197E-108.4779913
ACTAGGC6604.6933383E-78.36150411
CGTGGGT9102.4738256E-108.34141657
CCGGACA10052.5465852E-118.23689512
CGATACG5059.607662E-58.19838529
CACCCGG13700.08.0560614