FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4338214
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG2201.9099389E-1020.38448731
GTCGGTT3351.0913936E-1116.53691
CGACCGT2753.739842E-916.3075932
GTTCGAC3302.6284397E-914.63467829
GTCGTTT7500.014.3113081
GTCGAAT3052.2351196E-713.6226091
CGATCGG3255.055299E-712.7381741
CGTGCGA3801.902663E-812.71067537
TCGACGC3701.844146E-712.12050643
GTCCGTT5551.8189894E-1111.8533071
TACCGCG2050.003729174811.78062536
CGCATAT2651.5554787E-411.7591391
GCCGTAA3852.9453622E-711.6912641
GTCCGCT4451.29657565E-811.6710551
AGTTCGA4156.502887E-811.63694728
TCGAATA3003.9597846E-511.5065012
GCGATCG3601.7388065E-611.50026740
GTCCAGT18900.011.358181
CTCGACT3054.544671E-511.3521741
GTCCCGA6109.094947E-1211.3521741