FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_210_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4415418
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG2100.024.64077431
CGACCGT2655.456968E-1219.5266532
GTTCGAC2902.1827873E-1117.84311729
CGGGAAT4700.014.0004911
GTCCGTT5200.013.986321
GTCGTTT6000.013.8531171
GTCGAGA3801.2314558E-913.670841
TTCGACC3952.3283064E-913.10015930
GTCGCGT3154.742429E-612.093991
GTCCGAT5551.8189894E-1111.8562711
CGCTAAT2357.487744E-411.7898871
TCGACGC4207.6785E-811.49837743
CGATATA2200.006319558210.9760964
GTCGGTT3803.1797044E-610.9366721
GTCGGAA7600.010.9366721
AAGTTCG4753.589048E-810.89282927
CTATCGG2250.007468768410.73206159
CTCGACG5251.3829776E-810.51280242
GTCCGAA4652.9629155E-710.4270771
GCGACTA3005.0394115E-410.3576892