FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_209_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_209_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7625285
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT10700.012.25221649
TCGATTC12650.010.90876850
GATTCCG12600.010.67825652
TCTCGAT11000.010.6637448
ATTCCGT13200.010.19288153
TTCTCGA12650.010.09074347
CGATTCC14600.09.45177551
GCGTAGT7353.3105607E-109.3906823
TCACCCG16150.08.9693993
ATCGACT11250.08.5839916
TCGTCGG7757.6361175E-88.01298148
CGTGGGT17350.07.95480357
TTGCGTA8353.2250682E-87.848676716
TCGACGT4400.00186584067.8385183
TAGACCG4500.00231742047.667546758
CCGGACA17100.07.665032412
TTTCTCG16200.07.453140746
CTCACTA17550.07.2712387
TGCGTAG9554.4557964E-87.223685317
CCCGGAC17800.07.16953711