FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320657_209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320657_209_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7625285
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG4250.021.10375831
CGACCGT5000.017.93819632
GTTCGAC5300.016.92282529
TCGACGC6350.014.66723843
GTCGAGA6200.013.4076551
TCGCGTA3457.406197E-812.99937466
TTCGACC6750.012.77649230
GTCGCGA2050.0036214811.8270791
AGTTCGA7800.011.49816428
CGCGTAA3954.3054752E-711.35388467
GTCCGAT9250.011.2334411
CGCGAGT3702.4227538E-611.18762243
TCTCGAC8100.011.07361341
CGATCGG6008.185452E-1110.92454641
GTCGGTT7001.8189894E-1210.8857391
TCGCGCG3207.696166E-510.77974162
AAGTTCG8350.010.740827
GTCCGCT10450.010.6063751
GACCGTC9850.010.15633933
GTCGGAT9550.010.1551871