FastQCFastQC Report
Wed 8 Mar 2017
batch_1486769965663.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebatch_1486769965663.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000
Sequences flagged as poor quality0
Sequence length279-30134
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACG120.3No Hit
TTTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACG110.27499999999999997No Hit
CTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC90.22499999999999998No Hit
CGGTATGCTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAA70.17500000000000002No Hit
ATGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACG50.125No Hit
ACATGCTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC50.125No Hit
CTTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAAC50.125No Hit
TAATACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC50.125No Hit
CAATACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC50.125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTTC101.7922238E-4489.11256
TCGTGTA101.7922238E-4489.11251
GTAGCCG101.7922238E-4489.11253
AATACTT204.3692125E-9489.11252
GGTAGCC158.9120294E-7489.11252
GGTATGC251.33113645E-8391.292
GTGCTTC202.8122704E-6366.834383
GTATGCT202.8122704E-6366.834383
CGGTATG202.8122704E-6366.834381
CTTGTAC156.039837E-4326.0751
CATGCGC156.039837E-4326.0752
ATTACTT156.039837E-4326.0755
CGTTCCG156.039837E-4326.0759
CGTACTT156.039837E-4326.0753
CGTGTAC156.039837E-4326.0752
GCCAGCG156.039837E-4326.0751
TGTGCTT256.8552363E-6293.46752
CGTTCGT256.8552363E-6293.46759
ACATGCT256.8552363E-6293.46752
CCGTTCG256.8552363E-6293.46758