FastQCFastQC Report
Wed 8 Mar 2017
batch_1486767933723.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebatch_1486767933723.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000
Sequences flagged as poor quality0
Sequence length276-33630
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC160.4No Hit
CTTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAAC110.27499999999999997No Hit
ATGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC90.22499999999999998No Hit
CTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACG90.22499999999999998No Hit
CTTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACG60.15No Hit
TTTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGCT60.15No Hit
TTTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAAC50.125No Hit
TCGTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAAC50.125No Hit
TGGTATGCTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAAC50.125No Hit
GTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACG50.125No Hit
CGGTATGCTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAAC50.125No Hit
TCATACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC50.125No Hit
TCATGCTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACG50.125No Hit
TGGTATGCTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAA50.125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGCC101.801591E-4488.262481
ATTACTT101.801591E-4488.262484
CCGTACT101.801591E-4488.262481
CGGTCAG101.801591E-4488.262489
GGTATGC202.8318736E-6366.196872
GCGTACT202.8318736E-6366.196872
CAGCGTT156.0713885E-4325.508331
CATGCTT303.3360266E-8325.508332
GCGTTCA156.0713885E-4325.508335
TCACTTC156.0713885E-4325.508332
TCGTACT156.0713885E-4325.508331
TTCGGTC156.0713885E-4325.508337
TCGGTCA156.0713885E-4325.508338
GTATGCT256.903003E-6292.95753
TCATGCT256.903003E-6292.95751
TGCGTAC256.903003E-6292.95751
CTTCCGT357.1988325E-8279.007148
ATACTTC457.2577677E-10271.256963
TTGTACT301.4291811E-5244.131262
CATACTT301.4291811E-5244.131262