FastQCFastQC Report
Wed 8 Mar 2017
batch_1486765023677.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebatch_1486765023677.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000
Sequences flagged as poor quality0
Sequence length258-31433
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAAC130.325No Hit
CTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACG130.325No Hit
CTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC110.27499999999999997No Hit
CTTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACG110.27499999999999997No Hit
TTTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACG90.22499999999999998No Hit
TCATGCTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACG80.2No Hit
ATGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC70.17500000000000002No Hit
TTTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAAC70.17500000000000002No Hit
TCGTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAAC70.17500000000000002No Hit
TCATACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC60.15No Hit
AACGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAAC50.125No Hit
ACGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC50.125No Hit
TGGTATGCTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAAC50.125No Hit
AACGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACG50.125No Hit
CGGTATGCTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAAC50.125No Hit
TTTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGCT50.125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT101.811302E-4487.387486
GGTATGC101.811302E-4487.387482
GTATACT101.811302E-4487.387483
TCATGCT251.3549652E-8389.909971
CTGTACT400.0365.540621
TAATACT156.1040977E-4324.925021
TGCATGC156.1040977E-4324.925021
CGTACTT303.36604E-8324.925023
ACCGTTC156.1040977E-4324.925028
TGTACCG156.1040977E-4324.925025
GCGTACT156.1040977E-4324.925022
TGGTATG156.1040977E-4324.925021
TACCGTT156.1040977E-4324.925027
TGCGTAC156.1040977E-4324.925021
AATACTT301.4394474E-5243.693762
ATACTTC501.378794E-9243.693743
CATACTT200.0014447566243.693742
ACTTCGT1500.0227.44755
TGTACTT750.0227.44752
TACTTCG1400.0226.287054