FastQCFastQC Report
Wed 8 Mar 2017
batch_1486760695446.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebatch_1486760695446.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000
Sequences flagged as poor quality0
Sequence length265-30115
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC110.27499999999999997No Hit
TTTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAAC110.27499999999999997No Hit
CTTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACG100.25No Hit
TCATGCTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC80.2No Hit
TGGTATGCTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAAC80.2No Hit
TCATGCTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACG80.2No Hit
ATGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACG70.17500000000000002No Hit
CTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACG70.17500000000000002No Hit
TTTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACG60.15No Hit
TGGTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAAC50.125No Hit
TTTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACGC50.125No Hit
TCGTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAAC50.125No Hit
TGGTATGCTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAA50.125No Hit
TCGTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAA50.125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCGG101.7680095E-4491.33754
CGTAGCG101.7680095E-4491.33752
GCGTACT101.7680095E-4491.33751
CGGCCTT155.958274E-4327.558322
GTATGCT403.45608E-10307.085943
GGTATGC503.6379788E-12294.80252
GCTGCTT356.976916E-8280.764281
TATGCTT456.9849193E-10272.96534
CTGCTTC501.3133103E-9245.668752
CTTCGGT200.0014102586245.668755
GCCTTCG200.0014102586245.668754
CATGCGC200.0014102586245.668752
CGTACTT200.0014102586245.668752
GCGGCCT200.0014102586245.668751
CGGTATG200.0014102586245.668751
TGGTATG401.3580939E-7245.668751
GTACTTC1150.0234.98753
TACTTCG1250.0216.188494
CCTTCGT352.5782325E-5210.57325
CTGGTTC250.002750369196.534998