FastQCFastQC Report
Wed 8 Mar 2017
batch_1486757815353.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebatch_1486757815353.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000
Sequences flagged as poor quality0
Sequence length279-30226
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC150.375No Hit
CTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACG100.25No Hit
ATGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC80.2No Hit
TCATGCTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACG80.2No Hit
TCGTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAAC70.17500000000000002No Hit
TGGTATGCTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAAC70.17500000000000002No Hit
TGGTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAAC60.15No Hit
TTTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACG60.15No Hit
TTTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAAC50.125No Hit
CACGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACG50.125No Hit
CTTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACG50.125No Hit
TGGTATGCTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAA50.125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATGCT204.3346517E-9489.899961
TGGATCG101.7836035E-4489.899966
CATGCTT400.0367.4252
CCGTACT202.7942588E-6367.4251
TACTGGT202.7942588E-6367.4254
CACGTAC202.7942588E-6367.4251
AATACTT202.7942588E-6367.4252
CTGTACT156.0107996E-4326.61
GGATCGT156.0107996E-4326.67
ACTTCCG156.0107996E-4326.65
TAGCCGC156.0107996E-4326.61
AACATGC156.0107996E-4326.61
GGTATGC156.0107996E-4326.62
GTACTGG156.0107996E-4326.63
GTGTACT156.0107996E-4326.63
CAATACT156.0107996E-4326.61
CTGGTTC256.8113495E-6293.946
ACTGGTT256.8113495E-6293.945
ATGCTTC357.079689E-8279.942873
ATACTTC501.3369572E-9244.953