FastQCFastQC Report
Wed 8 Mar 2017
batch_1486755245269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebatch_1486755245269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000
Sequences flagged as poor quality0
Sequence length278-39374
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATGCTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACG100.25No Hit
CTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACG90.22499999999999998No Hit
TTTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACG90.22499999999999998No Hit
ATGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACG80.2No Hit
CTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC80.2No Hit
ATGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC70.17500000000000002No Hit
CTTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACG70.17500000000000002No Hit
TGGTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAAC60.15No Hit
TTTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAAC60.15No Hit
TCATACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC60.15No Hit
CTTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAAC50.125No Hit
TCATGCTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC50.125No Hit
TCGTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAAC50.125No Hit
TAATACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC50.125No Hit
CGGTATGCTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAA50.125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGCA101.7533966E-4492.699981
CGGTATA101.7533966E-4492.699981
ATGCCGT101.7533966E-4492.699987
TCGGTTC101.7533966E-4492.699989
GGTATAC202.7313763E-6369.5252
GTATACT202.7313763E-6369.5253
GGTACTT155.909052E-4328.466647
CTGTACT155.909052E-4328.466641
CGGTACT155.909052E-4328.466646
TCGGTAC155.909052E-4328.466645
GCATGCT256.6581233E-6295.619962
GGCCGTT200.0013986139246.349998
CCATGCT200.0013986139246.349991
ATCGTTT200.0013986139246.349998
ATCGGTA200.0013986139246.349994
TATACTT301.37850075E-5246.349994
ACATACT200.0013986139246.349991
TGGTATG200.0013986139246.349991
TGCCGTT200.0013986139246.349998
CATGCTT452.4099427E-7218.977782