FastQCFastQC Report
Wed 8 Mar 2017
batch_1486747335877.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebatch_1486747335877.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000
Sequences flagged as poor quality0
Sequence length248-30653
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC140.35000000000000003No Hit
CTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACG100.25No Hit
ATGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACG70.17500000000000002No Hit
ACATGCTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC70.17500000000000002No Hit
TTTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAACGC70.17500000000000002No Hit
GTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC70.17500000000000002No Hit
CTTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACG70.17500000000000002No Hit
TTTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGCT70.17500000000000002No Hit
TTTGTACTTCGTTCAGTTACGTATTGCTGTTTTCGCATTTATCGTGAAAC60.15No Hit
TCATGCTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACGC60.15No Hit
TTTGTACTTCGTTCAGTTACGTATTGCTGTTTCGCATTTATCGTGAAACG60.15No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGCT101.7784303E-4490.375032
TCATGCT251.3142198E-8392.300021
CTTGTAC155.9933757E-4326.916661
GTATGCT155.9933757E-4326.916663
GTACTGG155.9933757E-4326.916664
TTGTACT403.4924597E-10306.484382
CATGCTT503.6379788E-12294.2252
CAGCCGC256.785045E-6294.2251
CTTCGGT200.0014185626245.187525
CTACTTC200.0014185626245.187522
GCTGCTT200.0014185626245.187521
ACTTCGG200.0014185626245.187524
TATGCTT200.0014185626245.187524
CTGCATC200.0014185626245.187521
GCTACTT200.0014185626245.187521
CGCTTCG200.0014185626245.187525
CTGTACT352.5984787E-5210.16071
ATACTTC250.0027665556196.150013
GGTATGC250.0027665556196.150012
GCTTCGT756.002665E-11196.155